Enzymatic Synthesis of Butyl Ferulate by Silica-Immobilized Lipase in a Non-Aqueous Medium
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Enzymatic Synthesis of Butyl Ferulate by Silica-Immobilized Lipase in a Non-Aqueous Medium
Butyl ferulate was synthesized using a silica-immobilized commercial lipase (Steapsin) in dimethylsulfoxide (DMSO). Lipase-immobilized by surface adsorption onto silica pretreated with 1% glutaraldehyde showed 89% binding of protein. The esterification of butanol (100 mM) and ferulic acid (50 mM) by silica-bound biocatalyst was carried out at 45 ̊C for 6 h under shaking (120 rpm). The optimizati...
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ژورنال
عنوان ژورنال: Journal of Biomaterials and Nanobiotechnology
سال: 2011
ISSN: 2158-7027,2158-7043
DOI: 10.4236/jbnb.2011.24049